Robust activity marking ram
WebA robust activity marking system for exploring active neuronal ensembles. Sorensen AT, Cooper YA, Baratta MV, Weng FJ, Zhang Y, Ramamoorthi K, Fropf R, LaVerriere E, Xue J, … WebFigure 6. The RAM system in rats and flies. (a–d) RAM labels active neuronal ensembles in rats exposed to inescapable stress (IS). (a) Schematic timeline of the experimental procedure. Rats were injected with AAV-RAM-NLS-mKate2 and AAV-Ef1a-EGFP vectors in the medial prefrontal cortex (mPFC). After 10 days they were either subjected to IS or left …
Robust activity marking ram
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WebJan 12, 2024 · The RAM-HA-GFP viral construct was generated by insertion of a HA-tagged GFP expression cassette into the multiple cloning site (MCS) of rAAV-RAM (rAAV-RAM (rAAV-RAM-d2TTA::TRE-MCS-WPRE-pA) was a kind gift from ... we used the robust activity marking (RAM) system (Sorensen et al., 2016) that allowed WebDec 11, 2015 · In this study, we used a novel method to make activity recognition robust against deceptive behavior. We asked 14 subjects to attempt to trick our smartphone …
WebMay 1, 2024 · To label the neuronal ensemble activated during learning, we used the robust activity marking (RAM) system (Sørensen et al., 2016) that allowed learning-dependent … WebWe have developed a Robust Activity Marking (RAM) system that allows for the identification and interrogation of ensembles of neurons. The RAM system provides …
WebSep 23, 2016 · Known as Robust Activity Marking (RAM), the new system consists of a specially designed DNA sequence that is switched on by neuronal activity. Compared … WebFeb 28, 2024 · We utilized the robust activity marking (RAM; Sørensen et al., 2016) system to label ensembles of neurons within gustatory cortex (GC) that were active during …
WebApr 16, 2024 · Both P F-RAM and P N-RAM were robustly activated by membrane depolarization in cultured mouse hippocampal neurons (Figure 1 B; numerical data and …
WebFeb 28, 2024 · To test this, we virally expressed the robust activity marking (RAM) system in GC to label the conditioning-activated neuronal ensemble through the activity dependent expression of tdTomato (Sørensen et al., 2016; Wu et al., 2024). RAM labeling is inhibited by doxycycline (Dox); by removing Dox we could restrict labeling to a small window ... iphone 14 wallet case australiaWebJan 14, 2024 · A) Schematic representation of the RAM-based viral construct used for neuronal ensemble tagging and of the experimental design. B) Percentage of preference for displaced object during the object location test session. Dashed line (65%) represents the threshold applied to identify AI and AU mice. iphone 14 wallet case with strapWebJun 9, 2024 · However, the cellular processes and molecular mediators between engrams and microglia underlying forgetting are poorly understood. We utilized doxycycline (Dox) … iphone 14 wallet case menWebIn recent years, many novel technologies including TALEN/CRISPR-CAS9 mediated effective genome editing, optogenetics, tissue clearing coupled with advanced 3D microscopy, connectome analysis, and single cell transcriptomics, have been developed. iphone 14 wallet case purpleWebApr 8, 2024 · neural circuit activations, we incorporated robust activity marking (RAM) system, an optimized synthetic activity-regulated element with the immediate early gene (IEG) cFos minimal promoter, which can drive the expression of a reporter protein in response to sensory stimuli and epileptic seizure 24) (Figure 2A). RAM-dependent … iphone 14 wallpaper pcWebSep 23, 2016 · We have developed a Robust Activity Marking (RAM) system that allows for the identification and interrogation of ensembles of neurons. The RAM system provides unprecedented high sensitivity and selectivity through the use of an optimized… View PDF Save to Library Create Alert Cite Figures from this paper figure 1 figure 2 figure 3 figure 4 … iphone 14 wallet case womenWeb(b) The robust activity response of P RAM is a result of the combination of the four repeated RAM EM and FOS minimal promoter, which alone are respectively weakly responsive or... iphone 14 wallpaper goes black