Greengate cloning
WebApr 27, 2024 · An important prerequisite for a molecular toolkit is a universal and cost-effective cloning method that can be easily adopted by different laboratories. To this end, we generated a set of plasmids based on the GreenGate cloning system (Lampropoulos et al., 2013; Figure 1; Supplemental Table S1). This Golden Gate cloning system allows … WebGolden Gate cloning is one of the easiest cloning methods in terms of hands-on time, as digestion and ligation can be done in one 30-minute reaction. The destination vector and entry vector(s) are placed in a …
Greengate cloning
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WebGreenGate cloning is simple and efficient since it uses only one type IIS restriction endonuclease, depends on only six types of insert modules (plant promoter, N-terminal tag, coding sequence, C ... WebOct 12, 2024 · The hip1 coding sequence devoid of BsaI restriction sites for GreenGate cloning was synthesized by Biocat (Germany). For recombinant protein expression, the hip1 sequence was amplified without SP and cloned into the pET28a (+) expression vector (Addgene, USA), eventually having a His-tag at the N-terminus. Sequences for cysteine …
WebCloning is performed by pipetting in a single tube all plasmid donors, the recipient vector, a type IIS restriction enzyme and ligase, and incubating the mix in a thermal cycler. … WebMay 14, 2024 · The Golden Gate cloning method is an easy and straight forward procedure. Here, Lampropoulos et al. describe a variety of it designed for plant transgenesis, calling it cleverly GreenGate. The whole procedure is possible due to type IIS restriction enzymes. These enzymes cut at a defined number of nucleotides after the …
WebHome - PLOS WebJul 19, 2024 · The driver lines were generated employing the fast and flexible GreenGate cloning system (Lampropoulos et al., 2013) but are compatible with any vector/transgenic line in which the expression of an effector is under the control of derivatives of the pOp promoter element (Moore et al., 1998). An important feature of our driver lines is the ...
WebThis plasmid is designed for Golden Gate cloning using the BsaI enzyme. These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were …
WebFor this study, plasmid constructs were generated via GreenGate cloning . The protein-coding regions of MsSND1, ... For ectopic expression of transcription factors, the respective GreenGate construct was transformed into Agrobacterium tumefaciens ASE (pSOUP +). Selected transgenic clones were incubated in liquid LB medium containing the ... flint population 2020WebGreenGate is a simple and efficient cloning system for rapidly assembling plant transformation constructs . It is based on the Golden Gate method. Using the type IIS … greater phoenix chinese christian churchWebOct 15, 2024 · One cloning kit, termed GreenGate, was designed to facilitate rapid cloning of . constructs using commonly used elements of plant transformation vectors [16]. Assembly . greater phoenix chinese church chandler azWeband versatile cloning system for the generation of plant transfor-mation vectors, which we named GreenGate. The GreenGate system allows rapid and efficient assembly of six … flint population chartWebJul 12, 2024 · GreenGate - a novel, versatile, and efficient cloning system for plant transgenesis. PLoS One 8 , e83043 (2013). Article ADS Google Scholar greater phoenix digital library appWebThe characteristic of GreenGate cloning technology is sufficient to simultaneously construct a variety of effector expression vectors fused with different tags. On the basis of the abundant six modules provided by GreenGate toolkit, it is also possible to use empty modules to carry out modifications for specific research. The availability of ... greater phoenix convention \u0026 visitors bureauWebGreenGate Cloning Kit Description: GreenGate is a simple and efficient cloning system for rapidly assembling plant transformation constructs. It is based on the Golden Gate … greater phoenix digital library/libby