Cck8 thp-1
WebBioAssay record AID 1767130 submitted by ChEMBL: Antiproliferative activity against human THP-1 cells assessed as reduction in cell viability incubated for 72 hrs by CCK8 assay. WebMar 26, 2024 · CCK8 results indicated that over-expressed lncRNA NR104098 can inhibit cell proliferation of NB4 and THP-1 cells in a time-dependent manner . To evaluate the effect of lncRNA NR-104098 on proliferation in AML cell lines, the expression of the proliferation-associated protein ki67 was determined by immunofluorescence staining analysis ( …
Cck8 thp-1
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WebThe inflammatory stimulation assay was carried out based on Zhao’s method with slight modifications. 33 THP-1 macrophages were inoculated in a 96-well microplate with 1 × 10 5 /mL cell density and cultivated until cells adhered to the wall. Then cells were pretreated with CFP (100, 200, 300 μg/mL), stimulated by LPS (1.0 μg/mL) and hatched ... WebFeb 15, 2024 · To explore the importance of mTORC1 pathway in the synergistic anti-leukemia effect of Ara-C/metformin, MHY1485 (mTOR agonist) was added to treat HL-60 …
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WebProliferation of THP-1 cells and THP-1-derived macrophages. CCK-8 tests of (a) THP-1 cells and (b) THP-1-derived macrophages were assayed on Day 1, 3 and 6 in response to different Mg 2þ... WebApr 29, 2024 · (A) CCK-8 assay was performed to determine the viability of A549 cells co-cultured with various percentage concentrations of THP-1-derived macrophages (MΦ) (100:1, 10:1, 1:1). The cell proliferation of A549 group was used as the control. (B) The Cell-IQ continuous surveillance system was used to examine the growth of A549 cells.
WebJun 1, 2024 · After 5 days of culture, primary cortical neurons were stimulated with addition of varying concentrations of Aβ25-35 or CM obtained from THP-1 monocytes. 2.5. Detection of cell viability using cell counting kit-8 (CCK8) Cells for each treatment group were counted and seeded in 96-well plates at 100 μl/well in quintuplicate for each treatment …
WebJun 5, 2024 · A cell counting kit-8 assay (CCK-8), flow cytometry, Quantitative Real-Time PCR assay (q-PCR), Western blot assay and enzyme-linked immunosorbent assay (ELISA) were used to determine the effect of different treatments on cell proliferation, expression of surface phenotype marker and expressions and secretion of inflammatory cytokines. girft right procedure right placeWebTGF-βR1(Transforming Growth Factor Beta Receptor 1)是癌细胞表面与TGF-β1(Transforming Growth Factor Beta 1)结合的主要受体之一，除此之外，还有两类TGF-βR2和TGF-βR3。 girft recommendations nhsWeb瑞禧生物提供科研级Metronidazole-PEG-Glucose 甲硝唑-聚乙二醇-葡萄糖,我们一直致力于研发纳米靶向科研产品以满足国内广大科研院校及其他相关科研单位的需求。Metronidazole-PEG-Glucose 甲硝唑-聚乙二醇-葡萄糖试剂不能用于人体治疗、药物开发、和其他商业用途。如果需要采购Metronidazole-PEG-Glucose 甲硝唑-聚 ... fun and sun san benito txWebJun 1, 2024 · THP-1 cells were pre-treated with or without various concentrations of DHA in RPMI 1640 with 0 % FBS for 24 h, followed by 50 μM Aβ25-35 treatment for 3 d. CM … girft psychiatryWebFeb 3, 2024 · THP-1 is a human monocytic cell line derived from an acute monocytic leukemia patient, and these cells have the potential to differentiate into macrophages, … girft rheumatology national reportWebSep 3, 2024 · We found that in THP-1-derived macrophages, LPS obviously increased SVIL mRNA and protein expression. Inhibition of TLR4 by Resatorvid (Res) remarkably reversed the LPS-induced SVIL expression. ... However, CCK8 data showed that SVIL knockdown did not affect the cell viability compared with control group with or without PMA incubation ... girft report renal medicineWebHuman monocyte cell line THP-1 cells were obtained from Procell Life Science & Technology (Wuhan, China). RPMI-1640 was a suitable medium for THP-1 cells and was obtained from Gibco. THP-1 cells in FBS concentration and CO 2 incubator conditions were the same as ATDC5 cells. PMA (Phorbol 12-myristate 13-acetate, 25 ng/mL, Meilunbio, … fun and sun smart hane sun